Intracytoplasmic Sperm Injection (ICSI)

ICSI Treatment for Infertility

Intracytoplasmic Sperm Injection ICSI)

What is ICSI?
  • ICSI is an acronym for Intracytoplasmic Sperm Injection
  • A fancy way of saying "inject sperm into egg"
  • ICSI is a very effective method to fertilize eggs in the IVF lab after they have been aspirated from the female.
  • Its main use is for severe male infertility cases and where sperm is extracted surgically from the testes or epididymis. However it’s also used for certain other indications such as, advanced maternal age, moderate to severe endometriosis, poor ovarian reserve etc. ICSI involves the use of specialized micromanipulation tools and equipment and inverted microscopes that enable embryologists to select and pick up individual sperm in a specially designed ICSI needle. The needle is carefully advanced through the outer shell of the egg and the egg membrane - and the sperm is injected into the inner part (cytoplasm) of the egg. This usually results in normal fertilization in about 75- 85% of eggs injected with sperm. However, first the woman must be stimulated with medications and have an egg retrieval procedure so we can obtain several eggs for IVF and ICSI. The difference between IVF and ICSI, is in the procedure in the laboratory and the process for the patient in both treatments remains the same. At Xenith, we have state of the art equipment and we specialize in ICSI Treatment for infertility in Pune. We are also the only IVF Centre, to conduct all surgical sperm extraction, under a high magnification microscope thus helping the couple achieve a much higher pregnancy rate with ICSI in severe male factor infertility. Call us today to book you appointment at our ICSI treatment clinic.

Intracytoplasmic Sperm Injection (ICSI)

Who should be treated with intracytoplasmic sperm injection?

There is no "standard of care" in this field of medicine regarding which cases should have the ICSI procedure and which should not.

Some clinics use it only for severe male infertility, and some use it on every case. The large majority of IVF clinics are somewhere in the middle of these 2 extremes. Xenith being the best IVF centre in PCMC, Pune, carries out the correct diagnosis and only then suggests the right infertility treatment.

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    How is ICSI performed?
    • The mature egg is held with a specialized holding pipette.
    • A very delicate, sharp and hollow needle is used to immobilize and pick up a single sperm.
    • This needle is then carefully inserted through the zona (shell of the egg) and in to the center (cytoplasm) of the egg.
    • The sperm is injected in the cytoplasm and the needle is removed.
    • The eggs are checked the next morning for evidence of Normal fertilization

    The ICSI process differs from IVF only in the Laboratory. All the steps for the patient from the time of using hormonal injections to stimulate the production of multiple eggs till the embryo transfer remain the same for the patient. However in the Lab, the oocytes are subjected to ICSI instead of just leaving the sperms surrounding the egg and waiting for fertilization as happens in IVF.